The fifth Botswana HIV/AIDS Impact Survey (BAIS V) is a cross-sectional, household-based, nationally representative survey that will assess the prevalence of key HIV-related health indicators such as incidence, prevalence, viral load suppression, and risk behaviors and will describe uptake of key HIV prevention, care, and treatment services. This two-stage cluster survey of 13,545 randomly selected households in Botswana will include 28,789 eligible participants aged 15-64 years, of whom 24,154 are expected to participate in the interview; of these, 21,142 are expected to participate in blood draw and HIV testing. In addition, the survey expects 3,762 children aged 6 weeks to 14 years born from HIV-positive mothers and mothers of unknown HIV status to participate in the blood draw and HIV testing. The total number of expected participants in the blood draw and HIV testing is 24,904.